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1) Produce a box and whisker plot to compare the mean amounts of DNA in micrograms produced using the silica resin spin column method and the inorganic method.

Assessment Brief

  1. Produce a box and whisker plot to compare the mean amounts of DNA in micrograms produced using the silica resin spin column method and the inorganic method. NOTE: Data for this can be found in the Refer/Defer area of the Blackboard site. Submit a copy of your plot (you may upload it as a JPEG, Tiff or Picture file).

  2. Quoting group data from the laboratory practicals found in the Refer/Defer area of the Blackboard site please state which method YOU would recommend for DNA extraction and purification and explain why. Your answer should not exceed 250 words, please give at least one reference source.

  3. Write one, clear, concise, experimentally testable null hypothesis regarding the two different methods used in this module to extract and purify DNA (50 words maximum).

  4. According to the data issued on Blackboard, which method gives, on average, the highest yield of DNA?

    • Silica Resin Spin column method

    • Inorganic method

  5. According to the data issued on Blackboard, which method gives, on average, the purest DNA?

    • Silica Resin Spin column method

    • Inorganic method

  6. According to the data issued on Blackboard, which method gives the least consistent yield of DNA?

    • Silica Resin Spin column method

    • Inorganic method

  7. According to the data issued on Blackboard, which method is the most consistent with respect to the purity of DNA produced?

    • Silica Resin Spin column method

    • Inorganic method

  8. Use the data supplied in the Excel work book found in the Refer/Defer area of the Blackboard site to produce a chart showing a standard curve of DNA migration through a gel. Add one LINEAR line of best fit to the most appropriate data on the graph to create a tool that could be used for accurate interpolation (to find the size of unknown fragments on the same gel, based on their migration distance). NOTE: you will be relying on skills learned and practiced at level 4 to do this. Submit a copy of your graph (you may upload it as a JPEG, Tiff or Picture file)

  9. What is the role of GelRed® in Agarose gel electrophoresis of DNA fragments? Please select the most correct answer.

    • GelRed® is a nucleic acid dye which enables the measurement of DNA fragments

    • GelRed® is a nucleic acid stain which enables visualisation of DNA and RNA using a spectrophotometer

    • GelRed® is a tracker dye that facilitates visualisation of the movement of DNA through Agarose gels under UV light.

    • GelRed® is a nucleic acid stain which enables the visualisation of DNA and RNA under UV light

  10. What is the role of di deoxy DNTPs in a Sanger sequencing reaction? Please select the most correct answer.

  11. When ever they are randomly incorporated during a Sanger sequencing reaction di deoxy DNTPs terminate the extension of the new DNA molecule

    • di deoxy DNTPs are the `building blocks` that extend the new DNA molecules in a Sanger sequencing reaction

    • di deoxy DNTPs make it harder for DNA polymerase to extend the new DNA molecules in a Sanger sequencing reaction

    • di deoxy DNTPs make it easier for DNA polymerase to extend the new DNA molecules in a Sanger sequencing reaction

  12. What is the role of the positive control in an enzyme digest reaction? Please select the most correct answer.

    • The positive control in an enzyme digest provides evidence that all of the components were added to the reactions

    • The positive control in an enzyme digest reaction provides evidence that the enzyme is active and that it is cutting as expected

    • The positive control in an enzyme digest reaction enables the investigator to see the band sizes produced by the enzyme

    • The positive control in an enzyme digest reaction provides evidence that the reaction is not contaminated

  13. The gel image below shows 7 alleles, let’s call them 1-7 in order of size, with 1 being the smallest and 7 being the largest. In this sample of 11 individuals, which allele appears 4 times

  14. Imagine the unlikely case that these 11 individuals were truly representative of the population; what is the frequency (f) of allele 6? Give your answer as a percentage, rounded to one decimal place, do not include the % symbol

  15. If the frequency of those exhibiting the phenotype for an autosomal recessive condition caused by a single known mutation in a given population is 1/11254, what is the carrier frequency? Give your answer as a percentage, rounded to three decimal places, do not include the % symbol

  16. If the frequency of those homozygous for a specific mutation that causes a given autosomal recessive condition is 1/11254, in a particular population of 542,764 individuals, how many individuals are likely to be carriers of the causative mutation? Please give your answer rounded to a whole number

  17. If the frequency of those exhibiting the phenotype for an autosomal recessive condition caused by a single known mutation in a given population is 1/11254, what is the frequency of the wild type allele? Give your answer as a percentage, rounded to three decimal places, do not include the % symbol. The gel image above shows 7 alleles, let’s call them 1-7 in order of size, with 1 being the smallest and 7 being the largest. In this sample of 11 individuals, how many are homozygous at this locus? PLEASE SUBMIT YOUR ANSWER AS ONE WHOLE NUMBER

  18. Please describe Alu elements and give details of their possible effects in humans. 250 words maximum, please fully reference your answer 

  19. The image above relates to the SNP rs2494732, please explain what you see and why this SNP is of interest to biological scientists. 300 words maximum, please fully reference your answer.

  20. Please produce a Punnett square to show the following cross: X-linked gene for eye colour where brown is dominant and heterozygotes show green eyed phenotypes. The male has blue eyes and the female has green eyes. You may hand draw the image, or produce it using a computer, then save it and upload it as a JPEG, Tiff or Picture file to the relevant field in Blackboard

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DNA Extraction, Electrophoresis and Molecular Genetics Assignment Help

What Is This Molecular Genetics Assignment About?

This assessment introduces students to a range of core molecular biology and genetics techniques commonly used in research, diagnostic laboratories, and biotechnology industries. The assignment focuses on analysing experimental data generated from DNA extraction and purification procedures, interpreting molecular genetics results, and applying fundamental principles of population genetics, DNA sequencing, and inheritance.

Students are expected to demonstrate practical understanding of laboratory methods such as silica resin spin column and inorganic DNA extraction techniques, agarose gel electrophoresis, Sanger sequencing, enzyme digestion, and DNA fragment analysis. Alongside laboratory knowledge, learners must also apply statistical and graphical skills, interpret genetic data, calculate allele frequencies using population genetics principles, and communicate scientific findings clearly using appropriate academic referencing. Overall, the assignment aims to bridge practical laboratory experience with data interpretation and scientific reporting skills expected within modern biological and biomedical sciences.

Why Do Students Struggle With This Assignment?

Many students initially assume this assignment is simply a collection of short questions. However, it quickly becomes challenging because it requires knowledge from several different areas of molecular biology, genetics, statistics, and bioinformatics simultaneously. Learners often find it difficult to move between practical laboratory concepts, mathematical calculations, graph construction, and written scientific explanations within a single assessment.

A particularly common issue is interpreting laboratory-generated datasets correctly. Students may know how techniques such as DNA extraction or gel electrophoresis work in theory but struggle when asked to analyse real experimental results, identify trends, or justify recommendations based on statistical evidence. Tasks involving population genetics calculations, allele frequencies, Hardy-Weinberg principles, and interpretation of SNP data can also prove difficult, especially for students who are less confident with mathematical aspects of genetics.

Common Challenges Faced by Students

  • Constructing accurate box-and-whisker plots and standard curves using experimental datasets.

  • Interpreting DNA extraction results and comparing yield, purity, and consistency between methods.

  • Selecting the most appropriate statistical measures to support conclusions.

  • Applying Hardy-Weinberg calculations to estimate carrier frequencies and allele frequencies.

  • Correctly interpreting gel electrophoresis images and identifying allele distributions.

  • Explaining molecular techniques such as Sanger sequencing and enzyme digestion in sufficient scientific depth.

  • Writing concise, fully referenced scientific explanations within strict word limits.

  • Linking laboratory findings to broader biological significance.

Why Students Often Seek Additional Support

This assessment demands both theoretical understanding and practical data analysis skills. Students frequently discover that although they understand the laboratory procedures, presenting and interpreting scientific data in an academically rigorous way is considerably more demanding. Producing publication-style graphs, performing genetic calculations accurately, and explaining molecular concepts clearly can be particularly time-consuming.

At Assignment Experts, we regularly support students studying genetics, molecular biology, biomedical science, and biotechnology by helping them interpret experimental findings, structure scientific answers, develop clear data presentations, and ensure that all responses meet university assessment standards. Whether students need support with DNA extraction analysis, electrophoresis data interpretation, population genetics calculations, or scientific report writing, our subject specialists provide tailored academic assistance designed to improve both confidence and grades.

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Recent Papers

You are comparing two DNA extraction methods (silica resin spin column vs inorganic method) using lab data and identifying which gives better yield, purity, and consistency using graphs and basic statistical interpretation.

You mainly need a box-and-whisker plot and a standard curve graph, but your answers must also interpret the data clearly (not just show it).

Your recommendation should be based on yield, purity, and consistency of DNA. You should justify it using the actual lab data, not opinion.

A testable statement saying there is no significant difference between the two DNA extraction methods in terms of yield or purity.

Amelia

Clear breakdown of what each graph should show. Helped me understand how to compare both DNA methods properly without overthinking it.

United Kingdom

★★★★★
Thomas

Finally understood what they meant by “consistency of yield” after reading this. Saved me from writing something totally off topic.

United Kingdom

★★★★★
Frank

Good support for the gel electrophoresis section. The explanation of what to focus on in results was actually useful, not generic.

United Kingdom

★★★★★
Grace

The results actually matched my lab brief for once. Made the box plot task and interpretation way easier to structure.

United Kingdom

★★★★★
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