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Construct a standard curve (it will be a straight line) by taking the mean of the duplicates for each standard.

You are supplied with a set of data for each of the three colorimetric assays and a protein electrophoresis.  Guidelines on the interpretation are provided below.

For each colorimetric assay:

  1. Construct a standard curve (it will be a straight line) by taking the mean of the duplicates for each standard.
  2. Using this, determine the values of analyte for the patient sample and the quality control, again using the mean of the duplicate absorbances.  (The sample blanks will need to be deducted from absorbances for total and direct for the bilirubin assay.)
  3. Compare the quality control to its expected value to ascertain whether the assay is valid.  It should be within 10%.
  4. If the assay is valid, compare the results for the patient to the reference range in the practical booklet.
  5. Study the schematic electrophoretic pattern shown in Figure 1 and consider how it could be interpreted.
  6. Decide whether the patient results are normal or abnormal.
  7. Using the notes on the Patient Request Form, also provided, consider the meaning of the data.

For the electrophoresis compare the patient to the control serum and comment on the findings.  Also try to link the significance of these data with the other assays.

Write the results and a summary of the findings in your logbook.

Patient Request Form

Surname  Manning

Forename Christopher

DOB 16/02/1951

UR X881952

NHS

Address 49 Everest Close

                        Letchworth

Drs Name K A Anderson

Surgery Address  Princes Avenue Group Practice, 59 Princes Avenue, Letchworth

Clinical History

Weight loss

EtOH?

Tests requested

LFTs, γGT

 

     

Total Protein by Biuret

Sample

Concentration (g/L)

Absorbance 1

Absorbance 2

Standard blank

0.00

0.000

0.002

Standard 1

10.00

0.070

0.064

Standard 2

20.00

0.172

0.163

Standard 3

40.00

0.226

0.234

Standard 4

60.00

0.385

0.387

Standard 5

80.00

0.451

0.437

Standard 6

100.00

0.550

0.542

Patient

 

0.234

0.221

Quality control

 

0.275

0.263

 

 

Albumin by Bromocresol green

 

Sample

Concentration (g/L)

Absorbance 1

Absorbance 2

Standard blank

0.00

0.000

0.000

Standard 1

5.0

0.116

0.115

Standard 2

10.0

0.251

0.248

Standard 3

20.0

0.374

0.372

Standard 4

30.0

0.512

0.520

Standard 5

40.0

0.662

0.651

Standard 6

50.0

0.810

0.823

Standard 7

60.0

0.904

0.893

Standard 8

70.0

1.088

1.042

Patient

 

0.412

0.426

Quality control

 

0.521

0.518

Bilirubin by Jendrassik & Grof method

Sample

Concentration (µM)

Absorbance 1

Absorbance 2

Standard blank

0.000

0.000

0.001

Standard 1

10.0

0.030

0.032

Standard 2

20.0

0.055

0.049

Standard 3

35.0

0.092

0.097

Standard 4

55.0

0.158

0.162

Standard 5

75.0

0.202

0.200

Standard 6

90.0

0.266

0.252

Patient blank

 

0.006

0.005

Patient direct

 

0.015

0.016

Patient total

 

0.053

0.049

QC blank

 

0.002

0.001

QC direct

 

0.068

0.061

QC total

 

0.211

0.198

Figure 1:  schematic diagram of serum protein electrophoretic pattern for patient and quality control

Details of Quality Control Serum

Expected values for Quality Control Serum

Lot no: 428UE

Total protein:  47.5 g/L

Expiry Date: 2021-02

Albumin:  31.1 g/L

 

Direct bilirubin:  37.7 μmol/L

 

Total bilirubin:  91.2 μmol/L

Details on sample label:                 Patient name: Manning, Christopher

Date of birth: 16/02/1951


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